CRISPR-Cas9 sgRNA synthesis

by cdbiosynsis on Mar 5th, 2024 10:10 AM

[color=#000000][size=3][font=segoeui]CRISPR-Cas9 sgRNA synthesis refers to the use of molecular biology techniques to synthesize sgRNA (single guide RNA) molecules in the CRISPR-Cas9 system. CRISPR-Cas9 is a technology used for gene editing that can accurately modify DNA sequences. SgRNA is a key component of the CRISPR-Cas9 system, which can guide Cas9 enzymes to locate target DNA sequences and perform cleavage or repair. Therefore, synthesizing sgRNA is a necessary step for conducting CRISPR-Cas9 gene editing experiments. The synthesis of sgRNA is usually achieved through in vitro synthesis methods, using chemical synthesis or in vitro transcription.[/font][/size][/color]
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